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Santa Cruz Biotechnology fitc
Fitc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 326 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/donkey+anti+rabbit+igg+fitc/donkey+anti-rabbit+IgG-FITC/pmc13038874-75-7-15
Average 94 stars, based on 326 article reviews
fitc - by Bioz Stars, 2026-10
94/100 stars

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Incubation:

Article Title: Development of KEAP1-targeting PROTAC and its antioxidant properties: In vitro and in vivo.
Article Snippet: .. After rinsing with PBS-T (0.1% (v/v) Tween 20 in PBS), cells were blocked with 1% (w/v in PBS-T) BSA for 1 h, incubated with anti-NRF2 antibody (Proteintech, 16396-1-AP, IL, USA) (1:200 in 1% BSA) overnight at 4 ◦C, washed with PBS-T, and incubated with donkey anti-rabbit IgG FITC (Santa Cruz Biotechnology, SC-2090, 1:200 in 1% BSA) for 1 h at room temperature. ..

Article Title: Astrocyte Loss Augments Body Weight Through Reduction in Adipose Sympathetic Outflows.
Article Snippet: Emerging evidence indicates that astrocytes modulate energy metabolism and homeostasis.. However, one important but poorly understood element is the necessity of astrocytes in the control of body weight.. Here, we apply viral vectorassisted brainregion selective loss of astrocytes to define physiological roles played by astrocytes in the arcuate nucleus of the hypothalamus (ARH) and to elucidate the involved mechanism.

Article Title: Development of KEAP1-targeting PROTAC and its antioxidant properties: In vitro and in vivo
Article Snippet: .. After rinsing with PBS-T (0.1% (v/v) Tween 20 in PBS), cells were blocked with 1% (w/v in PBS-T) BSA for 1 h, incubated with anti-NRF2 antibody (Proteintech, 16396-1-AP, IL, USA) (1:200 in 1% BSA) overnight at 4 °C, washed with PBS-T, and incubated with donkey anti-rabbit IgG FITC (Santa Cruz Biotechnology, SC-2090, 1:200 in 1% BSA) for 1 h at room temperature. ..

Article Title: Carma3 Protects from Liver Injury by Preserving Mitochondrial Integrity in Liver Sinusoidal Endothelial Cells.
Article Snippet: Primary LSECs were plated on glass-bottomed dishes for 2 d. LSECs were fixed in 4% formaldehyde for 15 min and permeabilized in 0.1% Triton X-100 (Sigma-Aldrich) for 3 min, followed by blocking in 10% FBS for 1 h. LSECs were then incubated with anti-TOMM20 Ab (WH0009804M1, Sigma-Aldrich), anti-LC3 (anti microtubule-associated protein 1 light chain 3) Ab (PM036, MBL), anti-COX IV (ab153709, Abcam) or DYKDDDDK Tag Ab (2368, Cell Signaling Technology) overnight. .. After being washed by PBS, LSECs were incubated with donkey anti-rabbit IgG-FITC (F3016, Santa Cruz Biotechnology) and donkey anti-mouse IgG-Alexa Fluor 555 (A31570, Life Technologies) for 1 h, followed by staining with Hoechst (Beyotime) for 5 min. As for tetramethylrhodamine methyl ester (TMRM), annexin V, or propidium iodide (PI) staining, LSECs were incubated with Image-iTTM TMRM reagent (Invitrogen), annexin V, or PI (KeyGEN BioTECH) for 20 min, followed by Hoechst staining (Beyotime) for 15 min. .. Finally, all the samples were detected by a confocal microscope (LSM710, Zeiss).

Blocking Assay:

Article Title: Astrocyte Loss Augments Body Weight Through Reduction in Adipose Sympathetic Outflows.
Article Snippet: Emerging evidence indicates that astrocytes modulate energy metabolism and homeostasis.. However, one important but poorly understood element is the necessity of astrocytes in the control of body weight.. Here, we apply viral vectorassisted brainregion selective loss of astrocytes to define physiological roles played by astrocytes in the arcuate nucleus of the hypothalamus (ARH) and to elucidate the involved mechanism.

Staining:

Article Title: Carma3 Protects from Liver Injury by Preserving Mitochondrial Integrity in Liver Sinusoidal Endothelial Cells.
Article Snippet: Primary LSECs were plated on glass-bottomed dishes for 2 d. LSECs were fixed in 4% formaldehyde for 15 min and permeabilized in 0.1% Triton X-100 (Sigma-Aldrich) for 3 min, followed by blocking in 10% FBS for 1 h. LSECs were then incubated with anti-TOMM20 Ab (WH0009804M1, Sigma-Aldrich), anti-LC3 (anti microtubule-associated protein 1 light chain 3) Ab (PM036, MBL), anti-COX IV (ab153709, Abcam) or DYKDDDDK Tag Ab (2368, Cell Signaling Technology) overnight. .. After being washed by PBS, LSECs were incubated with donkey anti-rabbit IgG-FITC (F3016, Santa Cruz Biotechnology) and donkey anti-mouse IgG-Alexa Fluor 555 (A31570, Life Technologies) for 1 h, followed by staining with Hoechst (Beyotime) for 5 min. As for tetramethylrhodamine methyl ester (TMRM), annexin V, or propidium iodide (PI) staining, LSECs were incubated with Image-iTTM TMRM reagent (Invitrogen), annexin V, or PI (KeyGEN BioTECH) for 20 min, followed by Hoechst staining (Beyotime) for 15 min. .. Finally, all the samples were detected by a confocal microscope (LSM710, Zeiss).



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